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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: (NH4)NO3

Your search received 13 results.

514 BACTO MARINE BROTH (DIFCO 2216)

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
NaF 0.002 0.057
(NH4)NO3 0.002 0.02
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514a HALF STRENGTH BACTO MARINE BROTH

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
NaCl 9.725 166.41
MgCl2 2.95 30.984
Bacto peptone 2.5 -
Na2SO4 1.62 11.405
CaCl2 0.9 8.11
Yeast extract 0.5 -
KCl 0.275 3.689
NaHCO3 0.08 0.952
Fe(III) citrate 0.05 0.204
KBr 0.04 0.336
SrCl2 0.017 0.107
H3BO3 0.011 0.178
Na2HPO4 0.004 0.028
Na-silicate 0.002 0.016
NaF 0.001 0.029
(NH4)NO3 8.0e-4 0.01
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514b MEDIUM 514 plus additional salt

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
Agar 17.5 52.032
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
NaF 0.002 0.057
(NH4)NO3 0.002 0.02
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514c REACTIVATION WITH LIQUID MEDIUM 514

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
Agar 18 53.519
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
Na2CO3 0.16 1.51
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
NaF 0.002 0.057
(NH4)NO3 0.002 0.02
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Solutions:
  • 1000 ml 514. BACTO MARINE BROTH (DIFCO 2216)

514e MARINE BROTH (ANAEROB)

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
MgCl2 5.9 61.968
Peptone 5 -
Na2SO4 3.24 22.811
Yeast extract 1 -
KCl 0.55 7.378
L-Cysteine HCl x H2O 0.3 1.708
Na2S x 9 H2O 0.3 1.249
NaHCO3 0.16 1.905
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
NaF 0.002 0.057
(NH4)NO3 0.002 0.02
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Gas phase:
  • 100 % N2

514f MARINE BROTH (ROTH CP73)

Compound Conc. [g/L] Conc. [mM]
NaCl 19.4 331.964
Agar 15 44.599
MgCl2 8.8 92.427
Peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
NaF 0.002 0.057
(NH4)NO3 0.002 0.02
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514g HALF STRENGTH BACTO MARINE BROTH with 50% Seawater

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
NaCl 9.725 166.41
MgCl2 2.95 30.984
Bacto peptone 2.5 -
Na2SO4 1.62 11.405
CaCl2 0.9 8.11
Yeast extract 0.5 -
KCl 0.275 3.689
NaHCO3 0.08 0.952
Fe(III) citrate 0.05 0.204
KBr 0.04 0.336
SrCl2 0.017 0.107
H3BO3 0.011 0.178
Na2HPO4 0.004 0.028
Na-silicate 0.002 0.016
NaF 0.001 0.029
(NH4)NO3 8.0e-4 0.01
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760 MARINE THERMOCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
NaF 0.002 0.057
(NH4)NO3 0.002 0.02
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Gas phase:
  • 80 % N2
  • 20 % CO2

1173 MODIFIED MEDIUM 514

Compound Conc. [g/L] Conc. [mM]
NaCl 39.45 675.051
Bacto peptone 10 -
MgCl2 5.9 61.968
Yeast extract 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
NaF 0.002 0.057
(NH4)NO3 0.002 0.02
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1354 BACTO MARINE BROTH WITH NA-ACETATE

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
Agar 15 44.599
Na acetate 10 121.901
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
NaF 0.002 0.057
(NH4)NO3 0.002 0.02
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