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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Double distilled water

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403 BSK-MEDIUM

Compound Conc. [g/L] Conc. [mM]
Bovine serum albumin 35.025 -
Gelatine 9.797 -
HEPES buffer 4.199 17.62
Proteose peptone no. 2 3.499 -
Glucose 2.099 11.653
Proteose yeastolate 0.7 -
Tryptone peptone 0.7 -
Na-pyruvate 0.56 5.087
Sodium citrate 0.49 1.898
N-Acetylglucosamine 0.28 1.265
MgCl2 x 6 H2O 0.21 1.033
L-Glutamine 0.07 0.479
Rabbit serum - 31675
Double distilled water - -
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Solutions:
  • 1000 ml Solution A
  • 200 ml Solution B
  • 143 ml Solution C
  • 86 ml Solution D

792 PYROLOBUS FUMARII MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.723 13.393
KNO3 0.99 9.793
KH2PO4 0.495 3.638
KCl 0.327 4.383
Na2S x 9 H2O 0.149 0.618
NaBr 0.05 0.481
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.169
SrCl2 x 6 H2O 0.007 0.026
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
CoCl2 x 6 H2O 0.002 0.014
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 9.9e-4 0.007
FeSO4 x 7 H2O 9.9e-4 0.004
Sodium resazurin 5.0e-4 0.002
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.3e-4
AlK(SO4)2 x 12 H2O 1.8e-4 3.8e-4
H3BO3 9.9e-5 0.002
Na2SeO4 9.9e-5 5.2e-4
Na2MoO4 x 2 H2O 9.9e-5 4.1e-4
CuSO4 x 5 H2O 9.9e-5 4.0e-4
Na2WO4 x 2 H2O 9.9e-5 3.0e-4
Calcium pantothenate 9.9e-5 2.1e-4
Vitamin B12 9.9e-5 7.3e-5
p-Aminobenzoic acid 7.9e-5 5.8e-4
KI 5.0e-5 1.9e-4
D-(+)-biotin 2.0e-5 8.1e-5
Distilled water - -
Double distilled water - -
Solutions:
  • 989 ml Solution A
  • 10 ml Solution B
  • 1 ml Solution C
  • 10 ml Solution D
  • 1 ml Wolfe's mineral elixir
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % H2
  • 20 % CO2

811 ACTIVATED CARBON MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2HPO4 x 12 H2O 9 63.399
Activated carbon 5 -
NH4Cl 1.5 28.043
KH2PO4 1.5 11.023
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.02 0.136
Fe(NH4)citrate 0.001 -
Na2MoO4 x 2 H2O 9.0e-4 0.004
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
MnCl2 x 4 H2O 3.0e-5 1.5e-4
NiCl2 x 6 H2O 2.0e-5 1.5e-4
Na2SeO3 2.0e-5 1.2e-4
CuCl2 x 2 H2O 1.0e-5 5.9e-5
Distilled water - -
Double distilled water - -
Solutions:
  • 1 ml Trace element solution TS2

1135 HD-MEDIUM, 1:10 diluted, modified

Compound Conc. [g/L] Conc. [mM]
Agar 3.75 11.15
MES 0.488 2.497
Casein peptone 0.125 -
Yeast extract 0.063 -
Glucose 0.025 0.139
Double distilled water - -
Solutions:
  • 500 ml Solution A
  • 500 ml Solution B

1145 MS-MEDIUM (MODIFIED)

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 7 28.4
Elemental sulphur 5 -
NH4Cl 2 37.39
MgCl2 x 6 H2O 2 9.838
NaS2O3 x 5 H2O 2 -
CaCl2 x 2 H2O 0.8 5.442
NaOH 0.8 20
KCl 0.48 6.439
K2HPO4 x 3 H2O 0.3 1.722
Na2-EDTA x 2 H2O 0.005 0.015
CoCl2 x 6 H2O 0.002 0.012
ZnCl2 1.0e-3 0.007
MnCl2 x 4 H2O 1.0e-3 0.005
FeSO4 x 7 H2O 1.0e-3 0.004
AlCl3 x 6 H2O 4.0e-4 0.002
Na2WO4 x 2 H2O 3.0e-4 9.1e-4
NiSO4 x 6 H2O 2.0e-4 0.001
CuCl2 x 2 H2O 2.0e-4 0.001
H3BO3 1.0e-4 0.002
H2SeO3 1.0e-4 7.8e-4
Na2MoO4 x 2 H2O 1.0e-4 4.1e-4
Double distilled water - -
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Solutions:
  • 200 ml MS Buffer
  • 20 ml Solution A
  • 1.5 ml Solution B
  • 10 ml Solution C - Trace mineral solution (Ferguson and Mah, 1983)
Gas phase:
  • 4 % O2

1155 SME MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 27.7 473.99
Elemental sulfur 10 -
MgSO4 x 7 H2O 7 28.4
MgCl2 x 6 H2O 5.5 27.053
KH2PO4 1 7.348
CaCl2 x 2 H2O 0.75 5.102
KCl 0.66 8.853
Na2S 0.5 6.324
(NH4)2SO4 0.5 3.784
Yeast extract 0.4 -
NaBr 0.1 0.972
H3BO3 0.03 0.485
SrCl2 x 6 H2O 0.015 0.056
Double distilled water - -
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Gas phase:
  • 80 % H2
  • 20 % CO2

1231 HALOPLASMA CONTRACTILE MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 6.925 118.497
Starch 5 -
MgSO4 x 7 H2O 1.75 7.1
MgCl2 x 6 H2O 1.375 6.763
KH2PO4 0.5 3.674
Na2S x 9 H2O 0.5 2.082
CaCl2 x 2 H2O 0.375 2.551
Yeast extract 0.2 -
Peptone 0.2 -
KCl 0.15 2.012
NaBr 0.025 0.243
H3BO3 0.008 0.121
SrCl2 x 6 H2O 0.004 0.014
KI 1.3e-5 4.8e-5
Resazurin 5.0e-7 2.2e-6
Double distilled water - -
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Solutions:
  • 250 ml Artificial seawater

1282 SH SEAWATER MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 24.32 416.153
Agar 15 44.599
MgCl2 x 6 H2O 10 49.188
Na2SO4 4 28.161
HEPES 2.38 9.987
CaCl2 x 2 H2O 1.5 10.203
KCl 0.66 8.853
NaHCO3 0.2 2.381
Peptone 0.2 -
Yeast extract 0.1 -
KBr 0.1 0.84
H3BO3 0.025 0.4
SrCl2 0.024 0.15
NH4Cl 0.021 0.4
Na2MoO4 x 2 H2O 0.01 0.041
KH2PO4 0.005 0.04
NH4NO3 0.003 -
NaF 0.003 0.07
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 2 H2O 1.0e-4 -
ZnCl2 3.6e-5 2.6e-4
Nicotinic acid 2.5e-5 2.0e-4
Para-aminobenzoic acid 2.5e-5 1.8e-4
Pyridoxine hydrochloride 2.5e-5 1.2e-4
Lipoic acid 2.5e-5 1.2e-4
Thiamine hydrochloride 2.5e-5 7.4e-5
Riboflavin 2.5e-5 6.6e-5
Calcium pantothenate 2.5e-5 5.2e-5
Cyanocobalamine 2.5e-5 1.8e-5
NiCl2 x 6 H2O 2.4e-5 1.9e-4
Biotin 1.0e-5 4.1e-5
Folic acid 1.0e-5 2.3e-5
CuCl2 x 2 H2O 2.0e-7 1.2e-6
Double distilled water - -
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Solutions:
  • 1 ml Trace element solution
  • 1 ml Vitamin solution

1469 THERMOCHROMATIUM/ALLOCHROMATIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
MOPS 1.225 5.854
Na acetate 1.167 14.222
NaHCO3 1 11.904
Na2S x 9 H2O 0.75 3.123
KH2PO4 0.583 4.287
Sodium pyruvate 0.5 4.544
NH4Cl 0.467 8.724
NaCl 0.467 7.985
MgSO4 x 7 H2O 0.233 0.947
Yeast extract 0.1 -
CaCl2 x 2 H2O 0.088 0.595
EDTA 0.006 0.021
FeCl2 x 4 H2O 0.002 0.009
CoCl2 x 6 H2O 2.2e-4 0.002
Na2MoO4 x 2 H2O 2.2e-4 9.1e-4
MnCl2 x 4 H2O 1.2e-4 5.9e-4
ZnCl2 8.2e-5 6.0e-4
VOSO4 x 2 H2O 3.5e-5 1.8e-4
NiCl2 x 6 H2O 2.9e-5 2.3e-4
CuCl2 x 2 H2O 2.0e-5 1.2e-4
H3BO3 7.0e-6 1.1e-4
Na2WO4 x 2 H2O 2.3e-6 7.1e-6
NaHSeO3 2.3e-6 -
Double distilled water - -
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Solutions:
  • 700 ml Solution 1 Mineral Salts
  • 300 ml Solution 2 NaHCO3
  • 1 ml Trace elements

1560 MARINES PLANCTOMYCES MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
NaCl 11.735 200.804
MgCl2 x 6 H2O 5.32 26.168
HEPES 2.38 9.987
Na2SO4 1.96 13.799
N-Acetylglucosamine 0.8 3.616
CaCl2 0.715 6.443
MgSO4 x 7 H2O 0.594 2.41
KCl 0.345 4.628
Glucose 0.25 1.388
Peptone 0.25 -
Yeast extract 0.25 -
Nitrilotriacetic acid 0.2 1.046
NaHCO3 0.095 1.131
CaCl2 x 2 H2O 0.067 0.454
KBr 0.048 0.403
SrCl2 0.02 0.126
H3BO3 0.013 0.21
NaF 0.002 0.036
Na-Nitrilotriacetat 0.002 -
ZnSO4 x 7 H2O 0.001 0.004
MnSO4 x H2O 5.0e-4 0.003
FeSO4 x 7 H2O 5.0e-4 0.002
Na2MoO7O4 x 2 H2O 2.5e-4 -
Na-EDTA 2.5e-4 7.4e-4
ZnCl2 1.0e-4 7.3e-4
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Fe SO4 x 7 H2O 1.0e-4 3.6e-4
Co(NO3)2 x 6 H2O 1.0e-4 3.4e-4
Nicotinamide 5.0e-5 4.1e-4
H2SeO3 5.0e-5 3.9e-4
NiCl2 x 6 H2O 5.0e-5 3.9e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
Thiamine-HCl x 2 H2O 5.0e-5 1.3e-4
Riboflavine 5.0e-5 1.3e-4
D-Calcium pantothenate 5.0e-5 -
Biotin 2.0e-5 8.2e-5
Folic acid 2.0e-5 4.5e-5
Na2B4O7 x 10 H2O 1.8e-5 -
Na2MoO4 x 2 H2O 1.0e-5 4.1e-5
CuSO4 x 5 H2O 1.0e-5 4.0e-5
Na2WO4 x 2 H2O 1.0e-5 3.0e-5
AlK(SO4)2 x 12 H2O 1.0e-5 2.1e-5
Vitamin B12 1.0e-6 7.4e-7
Double distilled water - -
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Solutions:
  • 20 ml Mineral salts solution
  • 1 ml Trace element solution
  • 250 ml Artifical sea water
  • 5 ml Vitamin solution (double conc.)
  • 50 ml "Metals 44"
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