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DSMZ

DSMZ 465 . MINERAL MEDIUM PH 7.25

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Labrys methylaminiphilus DSM 16812

* Modification: Plus 10mM methylammoniumchloride

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 3-7 days aerobic 28 °C

Main sol. 465 #

ml
Solution occurs in 7 other media (first defined in Medium 465).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.50 g - -
KH2PO4 1.00 g - -
(NH4)2SO4 0.50 g - -
MgCl2 x 6 H2O 0.10 g - -
Ca(NO3)2 x 4 H2O 0.05 g - -
Trace element solution SL-4 1.00 ml - -
Methylammoniumchloride* 10.00 mM - -
Distilled water 1000.00 ml - -
1 pH 7.25
2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.

Medium 1 #

1000
Solution occurs in 20 other media (first defined in Medium 1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Agar , for solid medium 15 g 15 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Medium 220 #

1000
Solution occurs in 22 other media (first defined in Medium 220).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 15 g - -
Soy peptone 5 g - -
NaCl 5 g - -
Agar 15 g - -
Distilled water 1000 ml - -
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).

Medium 535 #

1000
Solution occurs in 18 other media (first defined in Medium 535).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459) 		30 g - -
Agar 20 g - -
Distilled water 1000 ml - -
1 pH 7.3
2 Autoclave at 121°C for 15 min.

Medium 830 #

1000
Solution occurs in 9 other media (first defined in Medium 830).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.50 g - -
Proteose peptone
(Difco no. 3) 		0.50 g - -
Casamino acids 0.50 g - -
Glucose 0.50 g - -
Starch
(soluble) 		0.50 g - -
Na-pyruvate 0.30 g - -
K2HPO4 0.30 g - -
MgSO4 x 7 H2O 0.05 g - -
Agar 15.00 g - -
Distilled water 1000.00 ml - -
1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Last modified: 01.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 47
Strains with modifications: 47
Labrys methylaminiphilus DSM 16812

  10 mM Methylammoniumchloride

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.5 24.655
KH2PO4 1 7.348
(NH4)2SO4 0.5 3.784
MgCl2 x 6 H2O 0.1 0.492
Ca(NO3)2 x 4 H2O 0.05 0.212
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Cultivation metadata from 47 strains BacDive

pH 8 - 10 2 strains
pH 4 - 6 1 strain
phyla 2 47 strains
oxygen aerobe 10 strains
oxygen obligate aerobe 7 strains
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