514f
514f .
MARINE BROTH (ROTH CP73)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Photobacterium carnosum DSM 105454
* Modification: Mix 1 volume medium 514 and one volume medium 535 (trypticase soy agar/broth)
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 15 °C
Main sol. 514f #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5.00 | g | 5 | - | |
| Yeast extract | 1.00 | g | 1 | - | |
| Fe(III) citrate | 0.10 | g | 0.1 | 0.408 | |
| NaCl | 19.40 | g | 19.4 | 331.964 | |
| MgCl2 | 8.80 | g | 8.8 | 92.427 | |
| Na2SO4 | 3.24 | g | 3.24 | 22.811 | |
| CaCl2 | 1.80 | g | 1.8 | 16.219 | |
| KCl | 0.55 | g | 0.55 | 7.378 | |
| NaHCO3 | 0.16 | g | 0.16 | 1.905 | |
| KBr | 0.08 | g | 0.08 | 0.672 | |
| SrCl2 | 34.00 | mg | 0.034 | 0.214 | |
| H3BO3 | 22.00 | mg | 0.022 | 0.356 | |
| Na-silicate | 4.00 | mg | 0.004 | 0.033 | |
| NaF | 2.40 | mg | 0.002 | 0.057 | |
| (NH4)NO3 | 1.60 | mg | 0.002 | 0.02 | |
| Na2HPO4 | 8.00 | mg | 0.008 | 0.056 | |
| Distilled water | 1000.00 | ml | - | - | |
| Agar , if required | 15.00 | g/l | 15 | 44.599 | |
| 545. TRYPTONE SOYA BROTH (TSB)* | 1000.00 | ml | - | - | |
| 1 Final pH should be 7.6 ± 0.2 at 25°C. Add 15.0 g/l agar for solid media. We recommend using the medium supplied by Carl Roth GmbH + Co. KG for Marinomonas mediterranea DSM 23531. | |||||
This solution originates from Medium 535b.
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 17.0 | g | 17 | - | |
| Soy peptone | 3.0 | g | 3 | - | |
| D(+)-Glucose | 2.5 | g | 2.5 | 13.877 | |
| NaCl | 5.0 | g | 5 | 85.558 | |
| K2HPO4 | 2.5 | g | 2.5 | 14.353 | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.3. | |||||
| 2 Medium 545 is the same as Caso Bouillon (Merck or Oxoid, Germany). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.4 - 7.8 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 19.4 | 331.964 |
| Agar | 15 | 44.599 |
| MgCl2 | 8.8 | 92.427 |
| Peptone | 5 | - |
| Na2SO4 | 3.24 | 22.811 |
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Cultivation metadata from 83 strains
| pH | 6 - 8 | 10 strains |
| pH | 4 - 6 | 9 strains |
| pH | 8 - 10 | 3 strains |
| pH | 10 - 12 | 1 strain |
| phyla | 5 | 88 strains |
| Show more | ||