514
514 .
BACTO MARINE BROTH (DIFCO 2216)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Aeropyrum pernix DSM 11879
* Modification: Add to the medium after autoclaving 1.00 g/l Na2S2O3 x 5 H2O from a stock solution sterilized by filtration. Check medium pH after autoclaving and adjust if necessary.
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 90 °C
Main sol. 514 #
ml
Solution occurs in 4
other media (first defined in Medium 514).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Bacto peptone | 5.00 | g | 5 | - | |
|
Yeast extract
(Bacto ) |
1.00 | g | 1 | - | |
| Fe(III) citrate | 0.10 | g | 0.1 | 0.408 | |
| NaCl | 19.45 | g | 19.45 | 332.82 | |
|
MgCl2
(anhydrous) |
5.90 | g | 5.9 | 61.968 | |
| Na2SO4 | 3.24 | g | 3.24 | 22.811 | |
| CaCl2 | 1.80 | g | 1.8 | 16.219 | |
| KCl | 0.55 | g | 0.55 | 7.378 | |
| NaHCO3 | 0.16 | g | 0.16 | 1.905 | |
| KBr | 0.08 | g | 0.08 | 0.672 | |
| SrCl2 | 34.00 | mg | 0.034 | 0.214 | |
| H3BO3 | 22.00 | mg | 0.022 | 0.356 | |
| Na-silicate | 4.00 | mg | 0.004 | 0.033 | |
| NaF | 2.40 | mg | 0.002 | 0.057 | |
| (NH4)NO3 | 1.60 | mg | 0.002 | 0.02 | |
| Na2HPO4 | 8.00 | mg | 0.008 | 0.056 | |
| Na2S2O3 x 5 H2O* | 1.00 | g/l | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water. | |||||
Metadata
| Last modified: | 16.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Taxonomic range: | Bacteria |
| Taxonomic range: | Phages |
| Medium type: | Complex medium |
| Final pH: | 7.6 |
| Edit in Medium builder | |
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 19.45 | 332.82 |
| MgCl2 | 5.9 | 61.968 |
| Bacto peptone | 5 | - |
| Na2SO4 | 3.24 | 22.811 |
| CaCl2 | 1.8 | 16.219 |
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Cultivation metadata from 2200 strains
| halophily | moderately halophilic | 31 strains |
| halophily | halophilic | 16 strains |
| halophily | slightly halophilic | 13 strains |
| halophily | halotolerant | 8 strains |
| halophily | non-halophilic | 2 strains |
| Show more | ||